Lyon come from behind in the other game in Group D to secure a 2-1 win in Prague.
Sparta scored first after eight minutes through a Tomas Jun header. Michael Essien levelled for Lyon and Syvlain Wiltord bagged the second late on.
Elsewhere:
Monaco 2-0 Deportivo
Olympiakos 1-0 Liverpool
Dynamo Kiev 4-2 Bayer Leverkusen
Real Madrid 4-2 Roma
Bayern Munich 4-0 Ajax
Juventus 1-0 Maccabi Tel-Aviv