Van der Sar will make his first team re-appearance in the game at Old Trafford on Saturday afternoon in place of England keeper Ben Foster.
"Edwin plays tomorrow," United boss Sir Alex Ferguson has confirmed.
"He had a reserve game 10 days ago and has come through that fine. "He has been training for quite a spell now but with a hand injury you don't take any chances and that game has given him a good confidence boost."
Rooney, meanwhile, has been ruled out of the game with the calf injury he picked up whilst on international duty with England. The United striker is also likely to sit out the Champions League game against CSKA Moscow on Wednesday night.
"Calf injuries can be troublesome," Ferguson added. "We are trying our best to get him fit for Wednesday but I have my doubts on that.
"We know how difficult these things can be so we will not be taking any chances with the Moscow trip.
"He should be fit for the Liverpool game.